Author(s):
Reena Meshram, F Shirin
Email(s):
trenameahrum114@gmail.com
Address:
School of Studies in Biotechnology, Pt. Ravishankar Shukla University, Raipur 492 010, India
Genetics and Plant Propagation Division, Tropical Forest Research Institute (Indian Council of Forestry Research & Education), PO - RFRC, Mandla Road, Jabalpur - 482 021.
Published In:
Volume - 29,
Issue - 1,
Year - 2016
Cite this article:
Meshram and Shirin (2016). In vitro Axillary Bud Proliferation of Azadirachta indica A. Juss. (Neem) and Comparison of Nitrate Reductase Activity among in vitro and in vivo Grown Leaves. Journal of Ravishankar University (Part-B: Science), 29(1), pp.194.
PP-F32
In vitro
Axillary Bud Proliferation of Azadirachta indica A. Juss. (Neem) and Comparison
of Nitrate Reductase Activity among in vitro and in vivo Grown Leaves
Reena Meshram and F Shirin
School of Studies in
Biotechnology, Pt. Ravishankar Shukla University, Raipur 492 010, India
Genetics and Plant Propagation
Division, Tropical Forest Research Institute (Indian Council of Forestry
Research & Education), PO - RFRC, Mandla Road, Jabalpur - 482 021
Corresponding author email:
trenameahrum114@gmail.com
[Received
12 January 2016, accepted 28 January 2016]
Abstract: Axillary bud
proliferation is eminent techniques of micropropagation. Neem (Azadirachta
indica A. Juss.), is evergreen multipurpose ethno-medicinal tropical tree
native to the Indian subcontinent. The tree is linked to the history of the
Indian way of life as a symbol of health; and traditionally been used for
antiseptic, contraceptive, antipyretic and antiparasitic purposes. The present
work aimed to study genetic variation among different populations of
Azadirachtaindica A. Juss. (Neem), viz., Raigad, Shahdol, Bilaspur, Chhindwara
and Gwalior, for in vitro axillary bud proliferation and nitrate reductase
activity, Different parameters of bud break and shoot regeneration on 4
treatments of plant growth regulators that was (0.SuM) 6-Benzylaminopurine,
0.SpM Kinetin, 0.5paM 6-Benzylaminopurine + 0.5 uM Kinetin and without hormone
were studied. Effects of PGRS were found to be statistically non-significant.
However, Significant variation was observed among the different populations of
Neem for sprouting %, number of shoots, number of nodes per shoot and total
number of nodes per nodal segment. Raigad population was found to be the best
population for almost all the parameters. On comparison against nitrate
reductase activity between five different populations of Neem under in vitro
and in vivo conditions, the enzyme activity was found to be higher in the in
vivo leaves than in in vitro leaves. In conclusion, the variation exists in the
in vitro response of different populations of Neem during bud break, culture
establishment and nitrogen assimilation.
Keywords: Genetic
Variation, Axillary Bud, Nitrate Reductase