Cite this article:
Chandrakar and Gupta (2016). Antibacterial Compound from Endophytic Streptomyces sp (K-RI) from Root of Abutilon indicum (L) Sweet. Journal of Ravishankar University (Part-B: Science), 29(1), pp.193-194.
PP-F31
Antibacterial
Compound from Endophytic Streptomyces sp (K-RI) from Root of Abutilon indicum
(L) Sweet
Sandhya Chandrakar and AK Gupta
School of Studies in Life
Science, Pt. Ravishankar Shukla University, Raipur 492 010, India
Corresponding author email:
sandhyachandrakar86@ gmail.com
[Received
5 January 2016; accepted 14 January 2016]
Abstract: Endophytic
actinomycetes are becoming popular for their bioactive molecules and are
relatively unexplored in Chhattisgarh. In the present investigation, a total of
nine endophytic Streptomyces sp. were isolated from Abutilon indicum (L.) sweet
through surface sterilization process. Isolate K-RI showed maximum
antibacterial activity against Gram-positive human pathogens B. cereus
MTCC-430, B. subtilis MTCC-441, S. aureus MTCC-96, S. epidermidis MTCC-435 and
clinical human pathogens B. cereus JNMC-1, B. subtilis JNMC-2 and S.
epidermidis JNMC-4. Based on morphology, biochemical and physiological
properties using PIB WIN software the isolate K-RI was identified as
Streptomyces phaeochromogenes with ID score of 0.97. Ten different basal
production medium were assessed for evaluation of a suitable medium for
antibiotic production. Glucose soybean meal broth medium showed a maximum
antibacterial activity and was used for further optimization studies. The
optimized culture condition for antibacterial compound production using central
composite design was as: Inoculum size-3.9 %, Incubation time-222 h, pH-7.62,
Temperature-30.25°C. In addition, optimization enhanced the antibacterial
activity spectrum also. The compound was extracted with ethyl acetate and
tested for purity by thin-layer chromatography. A single spot with Rf value of
0.66 was detected. Further purification using HPLC and characterization of the
active molecule is at hand.
Keywords: Endophytes,
Streptomyces, Phacochromogenes, Antibiotics, cCD, TLC