Cite this article:
Raghu (2016). Protective Effect of Boerhaavia diffusa L against Angiotensin II Induced Hypertrophy in H9c2 Cells. Journal of Ravishankar University (Part-B: Science), 29(1), pp.191-192.
PP-F28
Protective
Effect of Boerhaavia diffusa L against Angiotensin II Induced Hypertrophy in
H9c2 Cells
KG Raghu
Agroprocessing & Natural
Products Division, CSIR National Institute for Interdisciplinary Science &
Technology (NIIST), Thiruvananthapuram - 695 019, India
Corresponding author email: raghukgopal2009@
gmail.com
[Received
26 January 2016; revised version received January 2016; accepted IFabuary 2016]
Abstract: The present
study evaluated the antihypertrophic potential of the ethanolic extract of
Boerhaavia diffusa (BDE), a well- known edible cardiotonic plant reported in
Ayurveda against angiotensin Il- induced hypertrophy in H9c2 cardiac myoblast
cell. Markers of hypertrophy such as cell size, protein content and the
concentrations of atrial natriuretic peptide (ANP) and B-type natriuretic
peptide (BNP) were analysed for the confirmation of hypertrophy induction.
Angiotensin I1 (100nm) caused an increase in cell volume (69.26 (SD 1.21)%),
protein content (48.48(SD 1.64)%), ANP (81.90 (SD 1.22)%) and BNP (108.57 (SD
1.47)%). BDE treatment significantly reduced cell volume, protein content and
the concentrations of ANP and BNP (P0.05) in H9c2 cells. The activity of
various antioxidant enzymes and the concentration of reduced glutathione, which
was lowered duc to hypertrophy, were increased in BDE-treated cells. The BDE
treatment also reduced lipid peroxidation and protein carbonyls in cells. The
mitochondrial dysfunction during hypertrophy was evident from increased
intracellular ROS production (26.2520.91%), mitochondrial superoxide generation
(65.06±2.27%), alteration in mitochondrial transmembrane potential (AYm),
opening of mitochondrial permeability transition pore (MPTP) and mitochondrial
swelling. In addition, activities of mitochondrial respiratory chain complexes
(I-IV), aconitase, NADPH oxidase, thioredoxin reductase, and oxygen consumption
rate and calcium homeostasis were found altered. Treatment with BDE
significantly prevented the generation of intracellular ROS and protected
mitochondrial function and integrity. Overall results revealed the protective
effects of B. diffusa against hypertrophy in H9c2 cells and the present
findings may shed new light on the therapeutic potential of B. diffusa in
addition to its nutraceutical potentials.
Keywords:
Mitochondrial, Boerhaavia diffusa, Cardiotonic, Antihypertrophic