Author(s):
Rakhi Sarpate, Priyanka Bhaisare, Poonam Patil, Arti Shanware
Email(s):
poonampatil369@gmail.com
Address:
Gurunanak College of pharmacy, Deepak Nagar, Behind CP. Foundary, Nari Road, Nagpur 440 026, India
Rajiv Gandhi Biotechnology Center, Larminarayan Institute of Technology, Amravati Road, Nagpur - 440 026, India.
Published In:
Volume - 29,
Issue - 1,
Year - 2016
Cite this article:
Sarpate, Bhaisare, Patil and Shanware (2016). Evaluation of Anti-Microbial Activity of Flavonoid Rich Fraction of Aerial Parts of the Plant Bridelia retusa Spreng. Journal of Ravishankar University (Part-B: Science), 29(1), pp.190.
PP-F25
Evaluation of
Anti-Microbial Activity of Flavonoid Rich Fraction of Aerial Parts of the Plant
Bridelia retusa Spreng
Rakhi Sarpate, Priyanka Bhaisare,
Poonam Patil and Arti Shanware
Gurunanak College of pharmacy,
Deepak Nagar, Behind CP. Foundary, Nari Road, Nagpur 440 026, India
Rajiv Gandhi Biotechnology
Center, Larminarayan Institute of Technology, Amravati Road, Nagpur - 440 026,
India
Corresponding author email:
poonampatil369@gmail.com
[Received
15 January 2016, accepted 29 January 2016]
Abstract: The plant
Bridelia retusa spreng belonging to the family Euphorbiaceae is commonly known
as Patharphod, khaja has long been used in traditional system of medicine as
astringent, anthelmintic and in many skin infections. The bark with gingili oil
is much used as liniment in rheumatism. Several compounds including flavonoids,
lignans, polyphenols, alkaloids, triterpenes were isolated from these plants
& some of them interacts with most of the key enzymes. Various
pharmacological studies were carried out with the extracts & purified
compounds. The survey of literature has revealed that the flavonoid rich
fraction of the plant has not been investigated for the anti-microbiabacterial
activity. The acerial parts of B. retusawere collected from the local Nagpur
region. It was identified by the Department of Botany, RTM Nagpur University, Nagpur
with the help of authentic herbarium species. In the present investigationsthe
material was dried in shade and then coarsely powdered and was used for the
extraction of total flavonoid fraction. The dried acrial part was defatted with
the help of petroleum ether (60-80") & the marc was used to isolate
flavonoid rich fraction. The flavonoid rich fraction was evaluated
qualitatively for the presence of flavonoid with the help of preliminary
Phytochemical test and Thin Layer Chromatography. The fraction was then
evaluated for the anti-microbial activity with the help of Gram-positive, Gram-
negative bacteria and Fungi.
Keywords: Bridelia
retusa, Flavonoids, Thin Layer Chromatography, Anti-microbial activity