Cite this article:
Kar, Bahadur, Ahmmed, Goswami and Mukherjee (2016).Exploring Interaction Potential of Coriendrum Sativum Extract through Drug Metabolizing Enzyme Inhibition Study. Journal of Ravishankar University (Part-B: Science), 29(1), pp.189.
PP-F24
Exploring
Interaction Potential of Coriendrum Sativum Extract through Drug Metabolizing
Enzyme Inhibition Study
Amit Kar, Shiv Bahadur, SK Milan
Ahmmed, Debayan Goswami and Pulok K Mukherjee School of Natural Product
Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata
700 032, India
Corresponding author email:
naturalpreductm@gmail.com
[Received
23 January 2016; accepted 28 January 2016]
Abstract: The present
study was focused to evaluate the possible interaction potential of
standardized extract of Coriendrum sativum (CS) on drug metabolizing enzymes
through CYP450 inhibition study. CS is used as food and also in therapy in
Indian Systems of Medicine. CS and its bioactive compounds were studied for
evaluation of inhibition potential on pooled CYP450, as well as human CYP3A4
and CYP2D6. The plant extract was standardized through RP-HPLC (Waters 600, C
column) using linalool as marker. Methanol: Water (80:20 v/v) with 1% Acetic
acid was optimized as mobile phase and elute was detected at 210 nm. The
presence of heavy metal (Pb, Cd, As & Hg) in the medicinal plant was
determined by Atomic Absorption Spectroscopy. Interaction potential of the
standardized CS extract and phytoconstituent with pooled CYP450 were
significantly less (p<0.05: pc0.01: P<0.001) than standard known
inhibitor. In the present study, extract showed higher inhibition than their
single bioactive compound. The concentrations of heavy metals present in the
plant extract were within the permissible limit. From the above study, we can
conclude that the CS and linalool contributed negligible food-drug interactions
for the tested isozymes and may not possess any harmful effect upon their
therapeutic benefits.
Keywords: Coriendrum
sativum, RP-HPLC, Cytochrome P450, Food-drug interaction, Atomic absorption
spectroscopy