Cite this article:
Chandrakar and Keshavkant (2016). Nitric Oxide Attenuates the Adverse Impacts of Arsenic through Improved Proline, Protein and Sugar Contents, and Growth Attributes in Glycine max L. Journal of Ravishankar University (Part-B: Science), 29(1), pp.188-189.
PP-F23
Nitric Oxide
Attenuates the Adverse Impacts of Arsenic through Improved Proline, Protein and
Sugar Contents, and Growth Attributes in Glycine max L
Vibhuti Chandrakar and S
Keshavkant
School of Studies in
Biotechnology. Pt. Ravishankar Shukla University, Raipur - 492 010, India
Corresponding author email id:
skeshaykant@ gmail.com
[Received
21 January 2016; accepted 27 January 2016]
Abstract: The
protective effects of nitric oxide (NO) against arsenic (As)-induced metabolic
disturbances in Glycine max L have been investigated. Accumulated data clearly
approves that As exposure (10 and 50 uM) resulted in a declined growth of
Glycine max L. seedlings. Moreover, above treatments also triggered a series of
alterations in Glycine max L. with increased production of reactive oxygen
intermediates such as superoxide radical and hydrogen peroxide, and damage to
cellular membranes by producing malondialdehyde (one of the lipid peroxidized
cyto toxic products). Overall results revealed that exposure of As suppressed
growth and biomass accumulation, with altered protein, sugar and DNA contents,
hence led to oxidative injury. In addition, proline, a compatible solute,
accumulates under As stress. The concentration of sodium nitroprusside (0.02
mM, NO donor) was found to be quite effective in counteracting the adverse
effects of As exposure. NO has found to enhance growth traits, biomass
accumulation and restored oxidative stress markers. Therefore, it can be
concluded that As-induced abnormalities are partially or fully reversed by a
simultaneous application of As and NO donor and has an ameliorating effect
against As-induced oxidative stress in Głycine max L.
Keywords: Arsenic,
Glycine max L, Nitric oxide, Reactive oxygen intermediates, Sodium
nitroprusside