Author(s):
Roshan Ramteke, Simran Kukreja, Shruti Motwani, Arti Shanware
Email(s):
roshanramteke05@yahoo.com
Address:
Gurunanak College of pharmacy, Deepak Nagar, Behind C.P. Foundary, Nari Road, Nagpur 440 026, India
Rajiv Gandhi Biotechnology Center, Laxminarayan Institute of Technology, Amravati Road, Nagpur - 440 026, India.
Published In:
Volume - 29,
Issue - 1,
Year - 2016
Cite this article:
Ramteke, Kukreja, Motwani and Shanware (2016). Evaluation of Anti-Microbial Activity of Flavonoid Rich Fraction of Aerial Parts of the Plant Tephrosia purpurea Linn. Journal of Ravishankar University (Part-B: Science), 29(1), pp.182-183.
PP-F13
Evaluation of
Anti-Microbial Activity of Flavonoid Rich Fraction of Aerial Parts of the Plant
Tephrosia purpurea Linn
Roshan Ramteke, Simran Kukreja,
Shruti Motwani and Arti Shanware
Gurunanak College of pharmacy,
Deepak Nagar, Behind C.P. Foundary, Nari Road, Nagpur 440 026, India
Rajiv Gandhi Biotechnology
Center, Laxminarayan Institute of Technology, Amravati Road, Nagpur - 440 026,
India
Corresponding author email:
roshanramteke05@ yahoo.com
[Received
15 January 2016; accepted 28 January 2016]
Abstract: The plants
of the genus Tephrosia purpurea Linn are commonly called as Sharapunkha in
Ayurvedic system of medicine belonging to the family Fabaceac. The aerial parts
are widely used as anthelmintic, bitter-tonic, blood purifier etc. The plant
was found to be rich in flavonoids, rutin, and quercetin. Several compounds
including flavonoids, lignans, polyphenols, alkaloids, triterpenes were
isolated from these plants & some of them interacts with most of the key
enzymes. Various pharmacological studies were carried out with the extracts
& purified compounds. The survey of literature has revealed that the
flavonoid rich fraction of the plant has not been investigated for the
anti-microbial activity. The aerial parts of Tephrosia purpurea were collected
from the local Nagpur region. It was identified by the Department of Botany,
RTM Nagpur University, Nagpur with the help of authentic herbarium species. In
the present investigations the material was dried in shade and then coarsely
powdered and was used for the extraction of total flavonoid fraction. The dried
aerial part was defatted with the help of petroleum ether (60°-80°) & the
marc was used to isolate flavonoid rich fraction. The flavonoid rich fraction
was evaluated qualitatively for the presence of flavonoid with the help of
preliminary phytochemical test and Thin Layer Chromatography. The fraction was
then evaluated for the anti-microbial activity with the help of Gram-positive,
Gram- negative bacteria and Fungi.
Keywords: Tephrosia
purpurea, Flavonoids, Thin Layer Chromatography. Anti-microbial activity