Achla Jain, SD Belsare
School of Life Sciences, Pt. Ravishankar Shukla University, Raipur 492 010, India
Biochemistry Laboratory, Department of Bioscience, Barkatullah University, Bhopal 462 016, India.
Volume - 29,
Issue - 1,
Year - 2016
Cite this article:
Jain and Belsare (2016). Effect of Medicinal Plants Aqueous Extract on Drug-Induced Toxicity, In Vitro Study. Journal of Ravishankar University (Part-B: Science), 29(1), pp.179.
Medicinal Plants Aqueous Extract on Drug-Induced Toxicity, In Vitro Study
Achla Jain and SD Belsare
School of Life Sciences, Pt.
Ravishankar Shukla University, Raipur 492 010, India Biochemistry Laboratory,
Department of Bioscience, Barkatullah University, Bhopal 462 016, India
Corresponding author email:
14 January 2016, accepted 20 January 2016]
Abstract: Earlier in
vivo studies on rabbits and rats reported that the administration of sodium
salicylate, an active component of analgesic, antipyretic and anti-inflammatory
drugs like aspirin and paracetamol, induces toxic effects on liver and
erythrocytes due to reactive oxygen species generated due to this drug. The
present study was planned under in vitro condition to confirm these effects,
and evaluate antioxidant potential of medicinal plants (Withania Somnifera,
Curculigo orchioides and Tinospora cordifolia) in salicylate treated
erythrocytes considering changes in lipid peroxide (LPO) of membrane of
erythrocytes in series of in vitro assay. Lipid peroxidation (LPO) in
erythrocytes was determined in terms of malonyldialdehyde (MDA) formed as a
measure of oxidation of polyunsaturated lipids. It was quantified by measuring
malonyldialdehyde (MDA) produced using thiobarbituric acid (TBA) by the method
of Stocks and Dormandy (1971). The result showed that there was significant
(P<0.001) increase in LPO activity of erythrocytes exposed to sodium salicylate
at 10, 20 and 30 ppm concentration. Treatment with Withania Somnifera herb
extract at different concentration significantly (P0.001) lowers LPO activity.
In case of Curculigo orchioides and Tinospora cordifolia herb extracts LPO
activity was not significantly alters at lower concentration of extract but
significantly alters at higher concentration. It is concluded that the herb
extract has strong antioxidant property, which scavenges reactive oxygen
species (ROS) generated by sodium salicylate treatment in erythrocytes of goat
and these extracts could be a potential sources of natural antioxidant that
could have great important as therapeutic agents.
Keywords: In vitro
study, Lipid peroxidation, Sodium salicylate, Medicinal plants, Aqueous extract