Cite this article:
Dhurve and Vyas (2016). Combined Effect of Low Dose Acarbose and Ocimum tenuflorum for Treatment of Diabetes Induced Nephropathy in Rats. Journal of Ravishankar University (Part-B: Science), 29(1), pp.177.
of Low Dose Acarbose and Ocimum tenuflorum for Treatment of Diabetes Induced
Nephropathy in Rats
Preeti Dhurve and Amber Vyas
University Institute of Pharmacy,
P. Ravishankar Shukla University, Raipur 492 010, India
Corresponding author email:
4 January 2016; accepted 12 January 2016]
Abstract: The present
study investigated the combined effect of low doses Acarbose and Ocimum
tenuflorum ethanol leaf extract in diabetes-induced experimental nephropathy.
Rats were administered streptozotocin (35 mg/kg ip, once with high fat diet to
induce experimental diabetes mellitus. The development of diabetic nephropathy
was assessed biochemically and histologically. In addition, the lipid profile
and renal oxidative stress were assessed Lisinoprilwas used as the standard
drug for nephropathy. Acarbose was administered at 5 mg/kg as low dose and
Ocimum tenuflorum was administered at250mg/kg p.o.dose separately as well as in
combination. Statistical analysis was done by ANOVA followed by Dunnets
Multiple comparision test. P<0.05 was considered statistically significant.
Oral administration of combination of Acarbose and at 5 mg/kg p.o. and Ocimum
tenuflorum 250mg/kg respectively produced significant (p<0.01 & pc0.001)
decrease in lipid profile and oxidative stress. This combination of Acarbose
and Ocimum tenuflorum at low dose was more effective in attenuating the
diabetes-induced nephropathy and renal oxidative stress as compared to
treatment with either drug alone. It may be concluded that induction of
nephropathy in diabetic rats are due to diabetes-induced oxidative stress and
lipid profile alterations. The concurrent administration of Acarbose and Ocimum
tenuflorum at low doses may have prevented the development of diabetes-induced
nephropathy by reducing the lipid alteration, decreasing the renal oxidative
stress and certainly providing the direct nephroprotective action.
nephropathy, Ocimum tenuflorum, Combination therapy, Acarbose, Kidneys