Cite this article:
Sahu and Gawri (2016). A Comparative Study of Antibacterial Activity against Some Bacterial Stain between Different Parts of Ocimum Sanctum Tulsi. Journal of Ravishankar University (Part-B: Science), 29(1), pp.164-165.
PP-E05
A Comparative
Study of Antibacterial Activity against Some Bacterial Stain between Different
Parts of Ocimum Sanctum (Tulsi)
Bharti Sahu' and Shobha Gawri
Seth Phoolchand Agrawal Smriti College, Navapara, Rajim,
India
Corresponding author email:
bharti15august@ gmail.com
[Received
15 January 2016, accepted 29 January 2016]
Abstract: At present,
medical world is struggling with the resistance developed in microbial
pathogens against currently available antibiotics. Hence there is a great
necessity of novel antibiotics which can destroy the microbial pathogens.
Traditional medicinal plants are rich source of antimicrobial compounds. In
this context research on various extracts of Ocimum sanctum leaves have shown
significant results. Ocimum sanctum plants from surrounding area of Rajim Dist.
Gariyaband Chhattisgarh were selected for this study. Leaves stem and root of
Ocimum sanctum plants were first dried in shade at room temperature then
grounded to fine powder and stored at room temperature. Extracts were taken by
three methods viz methanol extracts, ethanolic extracts and aqueous extract.
Extracts from all the three types of extraction process showed antibacterial
activity against Gram positive bacteria like Streptococcus, Bacillus subtilis
and gram negative bacteria like Escherichia coli. Leaves of Ocimum sanctum
showed higher antibacterial activity in low concentration compare to stem and
root extracts. Methanol extracts showed significantly higher antibacterial
activity than extracts from other two types of extraction process. Minimum
inhibitory concentration data showed hopeful result as some of the extracts
exhibited significant inhibition of bacteria even at low concentration. The
zone of inhibition was measured in millimeter using vernier caliper. This study
indicate that leaves have significant higher antibacterial activity as compared
to stem and root of Ocimum sanctum and it could be very useful for developing a
novel antibacterial agents.
Keywords: Antibiotics,
Antibacterial activity, Ethanolic, Minimum inhibitory. Ocimum sanctum