Cite this article:
Banerjee, Harwnsh, Bahadur, Kar and Mukherjee (2016). Herb-Drug Interaction Potential and RP-HPLC Standardization of Trikatu - An Ayurvedic Formulation. Journal of Ravishankar University (Part-B: Science), 29(1), pp.161-162.
PP-DI5
Herb-Drug
Interaction Potential and RP-HPLC Standardization of Trikatu - An Ayurvedic
Formulation
Subhadip Banerjee, Ranjit K Harwnsh,
Shiv Bahadur, Amit Kar and Pulok Mukherjee
School of Natural Product
Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata
- 700 032, India
Corresponding author email:
ami.subhadipbanerjee@gmail.com
[Received
20 January 2016; accepted I February 2016]
Abstract: Trikatu is a
well known formulation in Ayurveda and most commonly used from ancient times in
India This formulation is consisted of three well known medicinal plants, viz.,
Piper longum (PL), Piper nigrum (PN) and Zingiber officinale (ZO) in equal
ratio. Trikatu has been used for the treatment of cough, cold, fever, asthma,
respiratory problems and improvement of digestive disorders. The main objective
in the present study was to investigate the effect of individual ingredients of
trikatu on drug metabolizing enzymes (CYP3A4 and CYP2D6), to find out its
herb-drug interaction potential through cytochrome P450 inhibition assays.
Additionally this work was aimed to develop a RP-HPLC method for the identification
and quantification of piperine and 6-gingerol in trikatu. Inhibition potential
of PL, PN and ZO were studied through CYP450-CO complex assay using rat liver
microsomes (RLM) and fluorescence screening method using individual isoenzymes
(CYP3A4 and CYP2D6). Quantification of piperine and 6-gingerol in LF, MF and
individual plant materials was performed through RP-HPLC method. Inhibitory
potential of trikatu formulation in CYP450-CO complex assay were found to be
and 35.12 2 2.31% (LF) and against CYP2D6 and CYP3A4 were estimated to be (IC50
251.30 z 3.98 and 245.23 1.92 He/ml) and (IC, 225.50 1.02 and 223.254 + 0.92
ug/mL) respectively. Trikatu formulation and its individual components showed
significantly (p < 0.001) less inhibitory activity on individual isoenzymes
as compared to the positive control. Outcome of the present study demonstrated
that trikatu has less interaction potential with drug metabolizing enzymes.
Keywords: Ayurveda,
Trikatu, Herb-drug interaction, Cytochrome P450, RP-HPLC