Cite this article:
Satnami, Patel, Vaishnav, Chandraker, Korram, Nagwanshi and Ghosh (2016). Silver Nanoparticles Based Spectroscopic Technique for the Determination of Medicinally Important Thiol Compounds. Journal of Ravishankar University (Part-B: Science), 29(1), pp.159-160.
PP-DI2
Silver
Nanoparticles Based Spectroscopic Technique for the Determination of
Medicinally Important Thiol Compounds
Manmohan L Satnami, Kuleshwar
Patel, Sandeep K Vaishnav, Kumudini Chandraker, Jyoti Korram, Rekha Nagwanshi
and Kallol K Ghosh
School of Studies in Chemistry,
P. Ravishankar Shukla University, Raipur 492 010, India
Department of Chemistry, Govt.
Madhav Science PG College, Ujain -456 010, India
Corresponding author email: manmohanchem@gmail.com
[Received
04 January 2016, accepted 12 January 2016]
Abstract: The
determination of thiol based biological molecules and drugs, such as cysteine
(Cys). glutathione (GSH). a- lipoic acid, and sodium 2-sulfanylethane
sulphonate in human plasma are becoming progressively more important, due to
exploration of their essential role in numerous biological pathways. Herein we
demonstrate the development of a highly sensitive colorimetric method for the
determination of medicinally important thiol drugs (I-IV), based on aggregation
of the citrate capped silver nanoparticles (Ag NPs). This was exploited from
high affinity of thiols towards the Ag NPs surface, which could tempt
replacement of the citrate shell by the thiolate shell of target molecules,
resulting in aggregation of the NPs through intermolecular electrostatic
interaction or hydrogen-bonding. Because of aggregation, the plasmon band at
around 396 nm decreases gradually, along with formation of a new red shifted
band. The calibration curves are derived from the intensity ratios of absorbance
at SAu wR sen vompound I-IV respectively, to the original wavelength of 396 nm.
The calibration curves display a linear relation in the range of 1uM-100 uM
(tor I), SuM-200 uM (for ) 0 M LO0M (for III) and 10 uM -150 pM (for IV),
respectively. The obtained detection limits (3a) were found to be 1.5 pM, 6.6
pM, 10.5 pM and 11.6 µM for compound I-IV, respectively. The proposed method
has been successfully applied for the detection of thiol compounds in different
kind of real samples.
Keywords: Silver
nanoparticles, Aggregation, Thiol compounds, Electrostatic interaction,
Detection limits