Cite this article:
Jain (2016). Chemical Fingerprint Analysis and Pharmacological Evaluation of Leaf of Lannea coromandelica Linn. Journal of Ravishankar University (Part-B: Science), 29(1), pp.153-154.
PP-D02
Chemical
Fingerprint Analysis and Pharmacological Evaluation of Leaf of Lannea
coromandelica Linn
Vishal Jain
University Institute of Pharmacy,
P. Ravishankar Shukla University, Raipur - 492 010, India
Corresponding author email:
vishaljain123@ gmail.com
[Received
4 January 2016; accepted 12 January 2016]
Abstract: WHO is
highly recommended the customary exploitation of traditional therapeutics of
medicinal plants and is consequently in pipeline for development of monograph
and exploring their uncultivated pharmacological properties. Presently, correct
identification of medicinal plants by phytochemical fingerprints is a speedy
tool to ensure reproducible quality of herbal drugs. In our study, the ethyl
acetate and methanol extract of leaves of Lannea coromandelica (L.
coromandelica) was subjected for chemical fingerprint as well therapeutic aid
as antifilarial agents. Ethyl acetate and methanolic extract of leaves of L
coromandelica was prepared using buchi speed extractor. High performance thin
layer chromatography (HPTLC) was employed to develop chemical fingerprint and
filariciadal activity of ethyl acetate and methanol extract was assessed by
motility inhibition and MTT reduction assay with concentrations range 1000 to
25ug/ml. The HPTLC analysis of ethyl acetate and methanol extract was carried
out using hexane: ethyl acetate: formic acid: methanol (5:4:1:0.5 v/v/v/v) for
fingerprinting and quantification ellagic acid and quercetin. The HPTLC method
was found to give compact spots for Rf = 0.54 and 0.51 ellagic acid; Rf = 0.91
and 0.87 quercetin for ethyl acetate and methanol extract respectively. The
HPTLC method was validated as per the ICH guidelines. Inhibitory concentration
(IC) for the ethyl acetate and methanol extract was found to be 284.5ug/ml and
312.3ug/ml respectively. In motility assay, complete inhibition of motility was
observed for all concentrations. Hence, our study could be valuable for
inventing strategies for quality control parameter and justifies the ethnic
uses of plant in folkloric medicines.
Keywords: WHO, Lannea
coromandelica, HPTLC, Filariciadal activity