Author(s):
Debayan Goswami, Rashmi Das, Durbadal Ojh, Amit Kar, Pulok K Mukherjee, Debprasad Chattopadhyay
Email(s):
debayansmusic@gmail.com
Address:
ICMR Virus Unit, ID & BG Hospital, General Block 4, First floor, Beliaghata, Kolkata - 700 010, India
School of Natural Product Studies, Jadavpur University, Kolkata - 700 032, India.
Published In:
Volume - 29,
Issue - 1,
Year - 2016
Cite this article:
Goswami, Das, Ojha, Kar, Mukherjee and Chattopadhyay (2016). Evaluation of Antiviral (HSV-1) Effcacy of the Standardized Extract of Boswellia serrata. Journal of Ravishankar University (Part-B: Science), 29(1), pp.136.
PP-B10
Evaluation of
Antiviral (HSV-1) Effcacy of the Standardized Extract of Boswellia serrata
Debayan Goswami, Rashmi Das',
Durbadal Ojha, Amit Kar, Pulok K Mukherjee and Debprasad Chattopadhyay
ICMR Virus Unit, ID & BG
Hospital, General Block 4, First floor, Beliaghata, Kolkata - 700 010, India
School of Natural Product
Studies, Jadavpur University, Kolkata - 700 032, India
Corresponding author email:
debayansmusic@gmail.com
[Received
23 January 2016 accepted 29 January 2016]
Abstract: Herpes
simplex virus (HSV) type I and type 2 causes a variety of diseases including
herpes labiles, keratoconjunctivitis, encephalitis, and herpes genitalis, along
with the lifelong latent infection in sensory nerve ganglia. The available
drugs for treating HSV infections have limited efficacy and frequently develop
drug-resistant viruses. Since time immemorial ethnomedicinal plants have been
used for diverse ailments including infectious diseases. Thus we have evaluated
the anti-HSV potential of methanol extract of Boswellia serrata against Herpes
Simplex Virus -1 (HSV-1) depending on its traditional usage in skin infections
and standardization of the same with the active phytoconstituent. The antiviral
activity of the extract and pure marker compound was determined by Cytotoxicity
(CCso), Cytopathicity and median effective concentration (ECso), through MTT
(3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) and plaque
reduction assay. Standardization was carried out using boswellic acid as the
standard marker by an isocratic RP-HPLC method (Waters 600), acetonitrile:
water (0.1 M acetic acid, pH 3.0) was used as the mobile phase. Interestingly
it was found that the crude methanol extract of B. serrata exhibited potent
antiviral activity against HSV-IF than its active phytoconstituent boswellic
acid. While in the methanol extract of B. serrata, the boswellic acid content
was found to be 1.06 0.03 % (w/w) by RP- HPLC method. Thus, this study revealed
that methanol extract of B. serrata and the active phyto-constituent (boswellic
acid) possesses potent anti-HSV activity.
Keywords: HSV-1, Bos
wellic acid, HPLC, MTT, Boswellia serrata