Cite this article:
Malti (2016). In Vitro Clonal Propagation of Superior Line of (Trichosanthes dioica, Roxb.) an Economically Important Plant. Journal of Ravishankar University (Part-B: Science), 29(1), pp.131-132.
PP-B03
In Vitro Clonal
Propagation of Superior Line of (Trichosanthes dioica, Roxb.) an Economically
Important Plant
Malti Devleela
biotechs, VIP Road, Anad Vihar,
Raipur 492 001, India
Corresponding author email: maltisimpy@gmail.com
[Received
15 January 2016, accepted 23 January 2016]
Abstract: Pointed
gourd (Trichosanthes dioica Roxb.) belongs to family Cucurbitaceae, common name
Parwal the plant is a perennial, dioecious, and grows as a vine. Traditionally
T. dioica is multiplied through seeds, stem cuttings and root cuttings.
Propagation from seeds is not advisable due to less germination percentage and
imbalanced male-female ratio. Protein and minerals were rich in fruits and are used
in traditional system of medicine since ancient times. Pharmacological
activates of T. dioica is haemagglutinating, antibacterial, antifungal,
hypoglycaemic. An efficient protocol was developed for micro- propagation of
superior line of an economically important, a perennial vegetable crop. Shoot
initiation of T. dioica derived from green house grown plants, nodal segment
(1.5-2 cm) were inoculated in culture tubes containing semisolid MS medium
supplemented with 0.5 mg/ BAP. After 4 week, the cultures were inoculated in
second medium for shoot multiplication containing MS medium supplemented with
1.0 mg/l BAP. The cultures showed significant increase in shoot length and
shoot number. Maximum (90%) shoot elongation with an average shoot length of
4.4+0.06 cm was observed at 1.0 mg/ BAP. To further enhance the number of
shoots per explants, mother tissue was repeatedly subculture on fresh shoot
induction medium after each harvest of newly formed shoots. Thus, by adopting
this strategy, an average of 53 shoots per explants could be obtained. After
the root formation, the in vitro raised plantlets removed from culture tubes,
washed thoroughly in running tap water and then were planted in small root
trainers containing vermiculate for hardening in green house under temperature
29+2°C and relative humidity 70%. After fifteen days of transfer, 75 percent
plants survived in the green house.
Keywords:
Trichosanthes dioica, Benzyladeninepurin, Murashig and Skoog. Rooting,
Hardening