Cite this article:
Dewanjee and Khanra (2016). Abroma augusta L (Malvaceae) Leaf Extract Attenuates Diabetic Nephropathy via Reducing Oxidative Stress and Inflammatory Responses. Journal of Ravishankar University (Part-B: Science), 29(1), pp.109-110.https://doi.org/
OP-F21
Abroma augusta
L (Malvaceae) Leaf Extract Attenuates Diabetic Nephropathy via Reducing
Oxidative Stress and Inflammatory Responses
Saikat Dewanjee and Ritu Khanra
Advanced Pharmacognosy Research
Laboratory, Department of Pharmaceutical Technology. Jadavpur University,
Kolkata 700 032, India
Corresponding author email:
s.dewanjee@yahoo.com
[Received
23 January 2016; accepted 27 January 2016]
Abstract: Abroma
augusta L. (Malvaceae) leaf is traditionally used to treat diabetes in India.
Therefore, current study was performed to evaluate the protective effect of
chemically standardized methanol extract of A. augusta leaves (AA) against type
2 diabetes mellitus (T2DM) and its associated nephropathy in experimental rats.
Antidiabetic activity of AA (100 and 200 mg/kg. p.o.) was measured in
streptozotocin-nicotinamide induced type 2 diabetic (T2D) rats. Fasting blood glucose
level and serum biochemical markers were measured. The redox status,
transcription levels of signal proteins (NF-KB and PKCS) and histological
studies of renal tissues were performed. Phytochemical screening of extracts
revealed the presence of taraxerol, flavonoids and phenolic compounds in the
AA. T2D rats showed significantly (p<0.01) elevated fasting blood glucose
level. Alteration in serum lipid profile (p < 0.01) and release of membrane
bound enzymes (p < 0.01) like lactate dehydrogenase and creatine kinase,
which ensured the participation of hyperlipidemia and cell membrane damage in
diabetic nephropathy. T2DM significantly (p < 0.05-0.01) altered the redox
status, decreased the intracellular NAD concentrations in renal tissues of
experimental rats. Investigating the molecular mechanism, activation different
PKC isoforms was observed in the renal tissue. T2D rats also exhibited an
up-regulation of NF-«B and increase in the concentrations of pro-inflammatory
cytokines (IL-IB, IL-6 and TNF-a) in the renal tissues. However, Oral
administration of AA per day could significantly (p < 0.05-0.01) reduce
hyperglycemia, hyperlipidemia, membrane disintegration, oxidative stress,
vascular inflammation and prevented the activation of oxidative stress induced
signaling cascades leading to cellular damage. Histological studies also
supported the protective characteristics of AA. The results suggested that, AA
could offer prophylactic role against diabetic nephropathy.
Keyword: Aatidiabetic,
streptorotocin, nephropathy, vascular inflammation, oxidative stress