Author(s):
M N Motiwala, R A Gupta, N G Dumore, U N Mahajan, K R Danao
Email(s):
meha24m@gmail.com
Address:
Assistant Professor, Dadasaheb Balpande College of Pharmacy, Besa, Nagpur, Maharashtra.
Published In:
Volume - 29,
Issue - 1,
Year - 2016
Cite this article:
Motiwala, Gupta, Dumore, Mahajan and Danao (2016). TLR-4 Mediated Wound Healing Activity of Coccinia indica on Burn Wound Model in Mice. Journal of Ravishankar University (Part-B: Science), 29(1), pp.106-107.https://doi.org/
OP-F16
TLR-4 Mediated
Wound Healing Activity of Coccinia indica on Burn Wound Model in Mice
MN Motiwala, RA Gupta, NG Dumore,
UN Mahajan and KR Danao
Assistant Professor, Dadasaheb
Balpande College of Pharmacy, Besa, Nagpur, Maharashtra
Corresponding author email: meha24m@gmail.com
[Received
20 January 2016, accepted I February 2016]
Abstract. The leaves of
Coccinia indica has been traditionally used by Indian village people in wound
healing, jaundice, hypocholestrolemia and inflammation. The phytoconstituents
of C. indica includes flavonoids and stilbenes. Wound healing is a complex
process that includes inflammation, tissue formation, and remodeling. In order
to scientifically prove the claimed utilization of the plant, the effects of
the extracts were investigated using burn wound model in mice including in vivo
antioxidant and antimicrobial activity. Healing was assessed by the rate of
wound contraction, period of epithelization and hydroxyproline content. The
antimicrobial activity of extract was also studied against bacterial and fungal
strain using agar dilution method. In vivo antioxidant activity was performed
to understand the mechanism of wound healing potency The result showed that C.
indica leaf extract has significant wound healing activity as evident from the
rate of wound contraction and epithelization. Hydroxyproline content was
correlative with the healing pattern observed. C. indica leaf extract treatment
promotes TLR-4 mediated upregulation of proinflammatory cytokines tnf-a during
early phase of wound healing, inhibit ROS accumulation and exhibit moderate
antimicrobial activity. We propose induction of cytokine production as one of
the mechanism for acceleration of wound healing by Cindica leaf extract which
may be due to flavonoids and stilbenes.
Keywords: TLR-4, Wound healing. Coccinia indica