Cite this article:
Panigrahy and Bhatt (2016). Target Guided Isolation and In-Vitro Antidiabetic Activity of Compounds Isolated from Hedychium coronarium Rhizome. Journal of Ravishankar University (Part-B: Science), 29(1), pp.104-105.
OP-F13
Target Guided
Isolation and In-Vitro Antidiabetic Activity of Compounds Isolated from
Hedychium coronarium Rhizome
Suchitraku Panigrahy and Renu
Bhatt
Department of Biotechnology, Guru
Ghasidas Vishwavidyalaya (A Central University), Bilaspur 495 009, India
Corresponding author email: panigrahysuchitra@gmail.com
[Received
14 January 2016, accepted 30 January 2016]
Abstract: Diabetes is a
chronic metabolic disease associated with severe complications. Despite of
progress in medicinal industry herbal medicines are preferred due to their
reducing side effects. Hedychiumcoronarium member of zingiberaceae family has
been reported as a medicine for various diseases. The present study was
undertaken to isolate and characterize the compounds from the extract of
rhizome of H.coronarium and to evaluate their efficacy on in-vitro models of
type-Il diabetes. Isolation of compounds from the active dichloromethane (DCM)
and ethyl acetate (EA) extract of rhizome of H.coronarium was achieved by
Silica gel column chromatography to yield different fractions. The bioactive
fractions were then subjected to further purification to obtain purificd compounds.
The antidiabetic properties of the compounds were evaluated in-vitro by a-
amylase and a- glucosidase enzyme assay. The isolated terpenes from the
fractions exhibited inhibition against a- amylase and a- glucosidase in
in-vitro correlating to reduce the plasma glucose level. The present
investigation suggests the use of the isolated terpenes for treatment of
type-ll diabetes in future to reduce the side effects of marketed drugs.
Keywords: Antidiabetic
a- amylase, a- glucosidase