Cite this article:
Mahobiya and Gupta (2015). Endophytic Fungi from Selected Herbs & Shrubs of Raipur and their L-asparaginase Producing Potentials. Journal of Ravishankar University (Part-B: Science), 28(1), pp. 51-59.
Journal of Ravishankar University-B, 28, 51-59 (2015)
from Selected Herbs & Shrubs of Raipur and their L-asparaginase Producing
Deepika Mahobiya* and A.K. Gupta
School of Studies in Life Science, Pt. Ravishankar Shukla University,
Raipur - 492 010, Chhattisgarh, India
*Corresponding Author: firstname.lastname@example.org
[Received 23 March 2015, revised version received 16 April 2015;
accepted 22 April 2015]
Abstract. L-asparaginase is a chemotherapeutic
agent that degrades L-asparagine present in blood and indirectly starve tumour
cell which lead to cell death. Endophytic fungi from medicinal plants are known
to produce pharmaceutical rortant metabolites and enzymes. In the present
investigation, eighty endophytic fungi were isolated from six medicinal he viz.
Aloe vera (L.) Burm. f., Abrus precatorius L., Asparagus racemosus Willd.,
Catharanthus roseus (L.) G. Don., Hemidesmus indicus (L.) R. Br., Plumbago zeylanica
L. and three medicinal shrubs, viz., Abutilon indicum (L.) Sweet, Adhatoda
vasica Nees and Ocimum sanctum L. Eleven genera of fungi including Acremonium,
Alternaria, Aspergillus, Aureobasidium, Bipolaris, Colletotrichum, Curvularia,
Fusarium, Paecilomyces, Penicillium, Pestalotiopsis, and seven etivity sterile
forms were identified. Out of eighty isolated endophytic fungi, forty four
isolates showed L-asparaginase activity through plate assay, while nineteen
isolates exhibited L-asparaginase activity upon submerged fermentation.
Notably, Fusarium species isolated from the roots of A. precatorius, A. vasica,
A. vera, C. roseus and H. indicus exhibited promising L-asparaginase activity.
Collerotrichum gloeosporioides isolated from the stem of A. racemosus and C.
roseus also exhibited good L-asparaginase activity. Fusarium solani (Mart.)
Sacc. NFCCI-3301(GR6) showed maximum L-asparaginase and selected for
optimization purpose. Modified Czapek Dox medium was selected as the best
production medium among the cight media. Maximum L-asparaginase activity was
recorded with 1.0% L-asparagine on third day of fermentation in the modified
Czapek Dox medium. Soyabean meal was found to be the best additional source of
nitrogen which increased production by two fold with F, solani. Potassium
nitrate was the second best source of nitrogen. Addition of different carbon
source in place of glucose did not affect L-asparaginase production.
Keywords: L-asparaginase; endophytic fungi:
Fusarium solani; optimization
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