Journal of Ravishankar University Vol. 27 No. B (Science) 2014  PP 63-68  ISSN 0970-5910

M.G. Roymon*,Rashmi Zankyani and Mabel Varghese

Department of Microbiology   and Biotechnology,  St. Thomas College, Bhilai,  Chhattisgarh

"Corresponding Author: mg1122_roymon@yahoo.co.in

[Received 25 March 2014; revised version received 31March2014;  accepted 9 April 2014)

Abstract. The present  study was evaluated  to bring out the diversity  of thcnnotolerant bacteria isolated  from river  Shivnath, vhich  is a major source of water for Durg-Bhilai region. Membrane filtration  was performed for isolating  bacteria from water ud  filter  was incubated at 44.5°C  for isolating   thermotolerant   bacteria.  Total  of I 3 bacterial   isolates viz.   Escherichia coli ,z=.5),  Salmonella sp, (1), Vibrio sp. (1),  Pseudomonas fluorescence (4),  Klebsiella sp. (2) were identified using biochemical nd morphological analysis.   Antibiotic resistance pattern performed for gentamycin,   streptomycin,   nalidixic  acid,  arnpicillin, .iloramphenicol,   co-trimoxazole,   ceftriaxone and tetracycline. The  highest   number  of isolates  (69.23%)   was resistant   to xlidixie  acid,  followed by co-trirnoxazole   (61.5%)    and  ampieillin (53.8%).  Least  resistance   was  observed for streptomycin 1%) and chloramphenicol    (23%).  Pseudomonas was found to be sensitive to all antibiotics.   Multidrug resistance  was shown y Pseudomonas  sp., Vibrio sp., Klebsiella sp., E. coli. Plasmid profiling   was performed for the presence  of resistance bands .ir  all  isolates.   Presence of high molecular weight  plasmid  bands  was noted  in all isolates   except   I   E.  coli isolate, indicating chromosome  mediated  resistance.

Keywords: Multidrug resistance, membrane filtration,  plasmid profiling,  thermotolerant  bacreria,  E.coli

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