Cite this article:
Johal and Chahal (1997). Effect of different concentrations of Molybdenum, Iron and Zinc on seed germinationand other characteristics of mungbean seedlings (Vigna. Radiate L.). Journal of Ravishankar University (Part-B: Science), 10(1), pp. 1-4.
Journal of Ravishankar University (Part-B: Science) 1997, 10(1), pp.1-4 ISSN 0970-5910
Effect of different concentrations of Molybdenum,
Iron
and Zinc on seed germinationand other
characteristics of mungbean seedlings (Vigna. Radiate L.)
R.K. Johal
and
V.P.S. Chahal
Deptt. of Microbilogy, Punjab
Agricultural University, Ludhiana-141 004.
MS: Received :2.4.96;
Revised. 11.8.97; Accepted. 26.8.97..
Abstract. Micronutrients
such as B. Cu. Fe. Zn.
Mn.
Mo. Cl and
Co play
a specific role in the
growth
and
development of plants.
Although there are
number
of
reports
showing
positive response of optimum concentrations
of
molybdenum. iron and zinc in increasing N2
fixation
and other
growth
characteristics of lguminous crops
(Sharma and Chahal. 1983; Ajakaiya, 1986: Saxena
and Rewari.
1991) yet no information is available on the optimum
concentration
of these micronutrients on the germination
of seeds.
Therefore, an in vitro experiment was conducted to find
out
an
optimum
concentration of Mo. Fe and Zn for seed
germination of mungbean.
Healthy seeds of mungbean( Vigna. radiata L.)
Var ML-267 were surface sterilized by dipping
in 0.1 % HgCl2 and alcohol (95%)
for 2 minutes and
3 minutes respectively. These
seeds were washed several times with sterile distilled water to remove
surface
sterilizing
agent. Surface
sterilized
seeds were
kept in different concentrations of Mo (0,2,5. 10 and
15 ppm), Fe(0,2,4,6,8
and
10 ppm) and Zn{0,2,5, 10, 15,and 20 ppm) for
15 minutes. Twenty seeds of each treatment
were then transferred
aseptically to Petri
plates
containing sterilized filter paper. Sufficient sterile
distillled
water was added into the Petri plates
whenever required
to keep the seeds moist.
G_erminationof seeds
was counted after ev.ery
24 hrs upto 7 days.
Observations
such as shoot
and root
length
of seedlings and changes
in their colour were recorded.
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