Cite this article:
Sharma and Chahal (1988). Effect of hydrogen uptake positive and negative strains of Rhizobium along with the different concentrations of nitrate on the Mungbean Vigna radiata L var. G-65. Journal of Ravishankar University (Part-B: Science), 1(1), pp. 27-32.
Effect of hydrogen uptake positive and negative
strains of Rhizobium
along with the different
concentrations of nitrate on the Mungbean Vigna radiata L var. G-65
P SHARMA and VPS CHAHAL
Department of Microbiology, Punjab Agricultural
University, Ludhiana-141 004
MS received 16 March 1988; revised 11 July
1988
Abstract: Pot culture experiment was conducted to study the
effect ot
Hydrogen uptake positive (Hup+) and negative (Hup-) strains of
Rhizobium along with the
different levels
of NO3- on mungbean (Vigna radiata L) var. G-65. Hup+ strains have
been found superior than that of Hup- strains in terms of all the growth characteristics and
N2 fixation. Hup+ strains (R-1 & R-2) showed the maximum
number and dry
weight of the nodules, leghaemoglobin, nitrogenase and hydrogenase
activities of nodules at
the 20 ppm level of NO3-, while the nitrate
reductase activity of leaves, dry weight of shoot and total N-uptake was maximum at
the 30 ppm level of
NO3-. Hup+ strain R-2 fixed more N2 in the presence of 20 ppm
NO3- than that of Hup+ strain R-1.
Keywords: Rhizobium, Pot culture, mungbean.
NOTE: Full version of this manuscript is available in PDF.