Anuma Singh, Mrinal Sharma, Latha Rangan, Aditya N Panda
Department of Biosciences and Bioengineering, Indian Institute of Technology, Guwahati - 781 039, India
Department of Chemistry, Indian Institute of Technology, Guwahati - 781 039, India.
Volume - 29,
Issue - 1,
Year - 2016
The objective of the current study was to develop an efficient method for the recovery of glabrin, a complex cyclic amino acid from seed oil of Karanja using liquid-liquid extraction. The structure of compound was characterized by various analytical and spectroscopy methods that involved HR-MS, FT-IR, XRD, and FESEM. The optimized geometry of glabrin molecule has been determined by the method of density functional theory (DFT) wherein, we have calculated the IR wavenumbers and compared them with experimental data. The calculated wavenumbers are in excellent agreement with the experimental values. Further, the effect of glabrin was evaluated using multiparamatric flow cytometry to assess the viability and physical heterogeneity within bacterial populations of E. coli enterotoxic and Staphylococcus aureus. Using flow analysis, it was found that membrane integrity and intracellular enzymatic activity provides an extensive evidence for revealing 'viable but non-culturable' (VBNC) state along with the active and dead subpopulation in the heterogenous sample. Moreover, molecular docking study was undertaken for detection and characterization of molecular interactions between bacterial proteins and glabrin. This is the first report with regard to glabrin characterization and its utility as pharmacophore using flow and molecular docking analyses.
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